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Description
Gel permeation chromatography employs a stagnant liquid present in pores of beads as the stationary phase, and flowing liquids as the stationary phase. The mobile phase flows between the beads of the column and out of the pores of beads. Gel permeation chromatography often used for analysis of polymers and determination of relative molecular weight. Furthermore, gel permeation chromatography measures molecular volume and shape function as defined by intrinsic viscosity. The size exclusion technique of gel permeation chromatography is used to separate and characterise polymers. It is a one-of-a-kind technique for determining the molecular weight distribution of an analyte using polymer characterisation. The polymer’s profile is significant because it affects physical qualities including brittleness, elasticity, and toughness in its final application. The premise behind the gel permeation chromatography system is that various components have variable affinity for the stationary phase (Column Packing material). Smaller components become caught in the pores of the packing material, resulting in a long retention duration.
